英国南安普顿代写作业:亲水和疏水药物

2019-03-22 05:51

近年来，由于亲水和疏水药物在肿瘤治疗中的持续释放，立方相纳米连接系统在药物传递研究中被广泛应用。目前，theranostic方法也成为癌症治疗和诊断的前沿命题，以更好地管理癌症。此外，靶向给药系统可用于提高药物和显影剂在靶部位的治疗指标。本研究通过包埋雷帕霉素(作为抗癌模型药物)和QD 605(作为显像剂)制备了HER 2抗体偶联立方相纳米粒(CPNPs)，目的是开发一种单次给药成像的theranostic纳米制剂。采用不同的方法对CPNPs进行了理化表征。通过红外光谱(FT-IR)和核磁共振氢谱(1H NMR)分析，确定了与CPNPs结合的抗体。体外研究表明，她的2个负载CPNPs的共轭雷帕霉素比天然雷帕霉素有更好的治疗效果，并且在她的2个阳性skbr3细胞系中，雷帕霉素负载CPNPs通过受体介导的内吞作用增强了细胞摄取。通过western blot分析，评价了颗粒系统在不同信号通路上的分子机制。通过体外光学成像，进一步阐明了上述系统作为成像方式的应用。因此，这些多功能的CPNPs有望成为未来癌症治疗和诊断的有效靶向药物传递系统。
英国南安普顿代写作业:亲水和疏水药物
Recently, cubic phase nanoparticulate systems are used as attractive vehicle in drug delivery research due to sustained release of both hydrophilic and hydrophobic drugs for cancer therapy. Currently, theranostic approach also emerged as frontier proposition for cancer therapeutics and diagnostics for better cancer management. Moreover, targeted drug delivery systems may be applied to increase the therapeutic index of the drugs and imaging agents at the targeted site. With an aim to develop a theranostic nanoformulation capable of drug delivery and imaging in a single shot, in the present study, HER 2 antibody conjugated cubic phase nanoparticles (CPNPs) were prepared by entrapping rapamycin (as a model anticancer drug) and QD 605 (as an imaging agent). The physicochemical characterisations of CPNPs were performed by different techniques. The antibody conjugated to CPNPs was confirmed by FT-IR and 1H NMR spectral analysis. In vitro studies were performed and demonstrated that, HER 2 conjugated rapamycin loaded CPNPs showed improved therapeutic efficacy than that of native rapamycin and rapamycin loaded CPNPs in HER 2 positive SKBr 3 cell line following enhanced cellular uptake through receptor mediated endocytosis. The molecular mechanisms of the particulate system on different signalling pathways were evaluated by western blot analysis. Further, the use of above system as an imaging modality was elucidated through optical imaging under in vitro system. Thus, these multifunctional CPNPs can be used as an effective targeted drug delivery system for cancer therapy and diagnosis in coming future.